ConfocalVRTM

Immersive 3D volume visualization for confocal and other microscopy image stacks

New to ConfocalVR? See How to Load Your Own Data on Meta Quest for supported file formats, step-by-step transfer from your desktop to the headset, and tips on tuning multi-channel images.

Before measurement, there is seeing.

A cell biologist studying a confocal stack may be looking for spatial relationships between proteins, organelles, and structures: where two markers seem close enough to deserve a closer look, where a signaling complex sits relative to a membrane, how a network winds through a volume.  But the specimen is three-dimensional, and the workflow keeps making it flat. So the researcher scrolls through slice after slice, building the 3D picture in their head. Each slice shows part of the story, but none quite answers the question: how are these structures actually arranged in space?  ConfocalVR turns the stack back into the volume it represents. The researcher can examine the specimen as an interactive 3D volume, inspect spatial relationships directly, and bring a sharper, testable hypothesis back to their analysis tools.  Because the best analysis does not begin with every possible measurement. It begins with a better question. Whether the question is co-localization, connectivity, morphology, or an unexpected feature in the volume, ConfocalVR helps researchers notice structure before they commit to the next measurement.

Originally developed for confocal microscopy, ConfocalVR also supports fluorescence, multiphoton, light-sheet, SEM, cryo-EM, X-ray, and other 3D microscopy stacks where spatial relationships matter.

How researchers use ConfocalVR

Outreach and public engagement. 
Show your work to non-specialists in a way they immediately understand. Two Quest headsets and a phone hotspot is a portable demo station — no laptop, no cables, no setup. Researchers have used ConfocalVR at science fairs, conferences, and public events to make complex cell biology accessible.

Teaching and training. 
Students learn spatial relationships in tissue and cells faster when they can fly through the data themselves. Load your teaching datasets once, share the room name, and run a class where every student sees the same specimen in 3D.

Collaboration. 
Share a virtual space with colleagues anywhere in the world. Both of you load the same dataset, join the same room, and examine the specimen together with voice chat — point, rotate, discuss. No screen sharing, no flat projections.

Presentations to funders and review panels. 
A 60-second VR demo communicates spatial complexity that takes ten slides to explain. Load your best data, hand them a headset, and let the work speak for itself.

Research exploration. 
Navigate multi-channel Z-stacks, adjust thresholds per channel, measure distances, and identify regions of interest in immersive 3D. Export measurements and views for publication.

What it does

3D volume rendering
Z-stacks rendered as navigable 3D volumes. Fly through tissue, rotate specimens, examine structures from any angle. Two-hand grab to position and scale.
Multi-channel support
Up to 30 channels with independent brightness, threshold, and opacity controls. Compute blend functions between channel pairs.
Collaborative sessions
Share a virtual space with colleagues anywhere — examine the same specimen together in real time. Click collaborate, enter a room name, and you’re in.
Measurement and analysis
Measure physical distances. Compute voxel intensity histograms. Mark and count objects.
Broad format support
TIFF, NIfTI, DICOM, PNG, JPG. Compatible with data from confocal, two-photon, SEM, cryo-EM, X-ray, AFM, STED, SIM, and other volumetric techniques.
Cloud data loading
Download from Google Drive, institutional servers, or any web source via Quest Browser. ZIP auto-extraction. No PC required.
Excluder tool
3D excluder objects to isolate regions. Select the best 2D view for publications.
4D time sequences
Display time-sequence stacks as short 30-frame movies.
Real-time parameter control
Transfer functions, brightness, contrast, clipping planes — all adjustable in real time.
Smart data handling

Typical research datasets load directly. Very large files are automatically subsampled to fit Quest memory (~30 million voxels). Progress feedback during loading.

Standalone on Meta Quest

Runs on Quest 2, Quest 3, and Quest Pro. No PC, no tether, no setup. $20 on the Quest Store. Ideal for previews, demos, teaching, collaboration, and small-to-mid-sized datasets.

Desktop version available

For larger datasets, a Windows desktop version handles up to 128 million voxels and loads directly from your filesystem. Available on Gumroad.

Who it’s for

Scientists, educators, and core facility managers working with 3D microscopy data.

If your instrument produces a Z-stack, ConfocalVR can render it — and help you show it to the people who need to understand it. Used at universities, research institutes, and public outreach events worldwide.

The immersive space provided by ConfocalVR includes display panels (left-to-right) for Channel Info, Analytics, Render Controls, 3D Image, and Channel Controls.

Publications

2018   |   Journal of Molecular Biology

ConfocalVR: Immersive Visualization for Confocal Microscopy

2018   |   Journal of Molecular Biology

Molecular Visualization on the Holodeck

Real-World Use Cases

The Use of ConfocalVR in Vascular Research

Dr. Craig J Daly from School of Life Sciences at University of Glasgow on the use of ConfocalVR.

ConfocalVR Key Use Cases at Erasmus

Dr. Gert Van Cappellen on the use of ConfocalVR
at Erasmus MC Optical Imaging Center in Rotterdam, NL

Collaborative Exploration of Confocal Data

Multiple researchers share a virtual space to examine the same 3D specimen together in real time — pointing, rotating, and discussing from anywhere in the world.

If you have a question

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